5VC8

Crystal structure of the WHSC1 PWWP1 domain

5VC8 の概要

• エントリーDOI: 10.2210/pdb5vc8/pdb
• 分子名称: Histone-lysine N-methyltransferase NSD2, dodeca-2-deoxy-nucleotide, poorly resolved by electron density, DNA (5'-D(P*CP*TP*(DN))-3'), ... (5 entities in total)
• 機能のキーワード: pwwp domain, structural genomics consortium, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus . Isoform 4: Cytoplasm: O96028
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 44130.07

構造登録者

Qin, S.,Tempel, W.,Dong, A.,Bountra, C.,Weigelt, J.,Arrowsmith, C.H.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2017-03-31, 公開日: 2017-06-28, 最終更新日: 2024-10-23)

主引用文献

Zhang, M.,Yang, Y.,Zhou, M.,Dong, A.,Yan, X.,Loppnau, P.,Min, J.,Liu, Y.
Histone and DNA binding ability studies of the NSD subfamily of PWWP domains.
Biochem.Biophys.Res.Commun., 569:199-206, 2021

PubMed Abstract: The NSD proteins, namely NSD1, NSD2 and NSD3, are lysine methyltransferases, which catalyze mono- and di-methylation of histone H3K36. They are multi-domain proteins, including two PWWP domains (PWWP1 and PWWP2) separated by some other domains. These proteins act as potent oncoproteins and are implicated in various cancers. However the biological functions of these PWWP domains are still largely unknown. To better understand the functions of these proteins' PWWP domains, we cloned, expressed and purified all the PWWP domains of these NSD proteins to characterize their interactions with methylated histone peptides and dsDNA by quantitative binding assays and crystallographic analysis. Our studies indicate that all these PWWP domains except NSD1_PWWP1 bind to trimethylated H3K36, H3K79 peptides and dsDNA weakly. Our crystal structures uncover that the NDS3_PWWP2 and NSD2_PWWP1 domains, which hold an extremely long α-helix and α-helix bundle, respectively, need a conformation adjustment to interact with nucleosome.

PubMed: 34271259
DOI: 10.1016/j.bbrc.2021.07.017

実験手法

X-RAY DIFFRACTION (1.8 Å)