5VAW

Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075

5VAW の概要

• エントリーDOI: 10.2210/pdb5vaw/pdb
• 関連するPDBエントリー: 4XA2 5CFV 5IHJ
• 分子名称: Maltose-binding periplasmic protein,Type IV pilin PilA, (4S)-2-METHYL-2,4-PENTANEDIOL, SODIUM ION, ... (7 entities in total)
• 機能のキーワード: cell adhesion, type iv pilin
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54675.21

構造登録者

Piepenbrink, K.H.,Sundberg, E.J. (登録日: 2017-03-28, 公開日: 2018-03-28, 最終更新日: 2024-11-13)

主引用文献

Ronish, L.A.,Lillehoj, E.,Fields, J.K.,Sundberg, E.J.,Piepenbrink, K.H.
The structure of PilA fromAcinetobacter baumanniiAB5075 suggests a mechanism for functional specialization inAcinetobactertype IV pili.
J. Biol. Chem., 294:218-230, 2019

PubMed Abstract: Type IV pili (T4P) are bacterial appendages composed of protein subunits, called pilins, noncovalently assembled into helical fibers. T4P are essential, in many bacterial species, for processes as diverse as twitching motility, natural competence, biofilm or microcolony formation, and host cell adhesion. The genes encoding type IV pili are found universally in the Gram-negative, aerobic, nonflagellated, and pathogenic coccobacillus , but there is considerable variation in PilA, the major protein subunit, both in amino acid sequence and in glycosylation patterns. Here we report the X-ray crystal structure of PilA from AB5075, a recently characterized, highly virulent isolate, at 1.9 Å resolution and compare it to homologues from strains ACICU and BIDMC57, which are C-terminally glycosylated. These structural comparisons revealed that PilA exhibits a distinctly electronegative surface chemistry. To understand the functional consequences of this change in surface electrostatics, we complemented a Δ knockout strain with divergent genes from ACICU, BIDMC57, and AB5075. The resulting transgenic strains showed differential twitching motility and biofilm formation while maintaining the ability to adhere to epithelial cells. PilA and PilA, although structurally similar, promote different characteristics, favoring twitching motility and biofilm formation, respectively. These results support a model in which differences in pilus electrostatics affect the equilibrium of microcolony formation, which in turn alters the balance between motility and biofilm formation in .

PubMed: 30413536
DOI: 10.1074/jbc.RA118.005814

実験手法

X-RAY DIFFRACTION (1.69 Å)