5V7Q

Cryo-EM structure of the large ribosomal subunit from Mycobacterium tuberculosis bound with a potent linezolid analog

5V7Q の概要

• エントリーDOI: 10.2210/pdb5v7q/pdb
• 関連するPDBエントリー: 5V93
• EMDBエントリー: 8641 8645 8646 8647 8648
• 分子名称: 50S ribosomal protein L32, 50S ribosomal protein L3, 50S ribosomal protein L4, ... (32 entities in total)
• 機能のキーワード: rna dynamics, ribosome
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 31
• 化学式量合計: 1458448.81

構造登録者

Yang, K.,Chang, J.-Y.,Cui, Z.,Zhang, J. (登録日: 2017-03-20, 公開日: 2017-09-20, 最終更新日: 2024-03-13)

主引用文献

Yang, K.,Chang, J.Y.,Cui, Z.,Li, X.,Meng, R.,Duan, L.,Thongchol, J.,Jakana, J.,Huwe, C.M.,Sacchettini, J.C.,Zhang, J.
Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.
Nucleic Acids Res., 45:10884-10894, 2017

PubMed Abstract: Ribosomes from Mycobacterium tuberculosis (Mtb) possess species-specific ribosomal RNA (rRNA) expansion segments and ribosomal proteins (rProtein). Here, we present the near-atomic structures of the Mtb 50S ribosomal subunit and the complete Mtb 70S ribosome, solved by cryo-electron microscopy. Upon joining of the large and small ribosomal subunits, a 100-nt long expansion segment of the Mtb 23S rRNA, named H54a or the 'handle', switches interactions from with rRNA helix H68 and rProtein uL2 to with rProtein bS6, forming a new intersubunit bridge 'B9'. In Mtb 70S, bridge B9 is mostly maintained, leading to correlated motions among the handle, the L1 stalk and the small subunit in the rotated and non-rotated states. Two new protein densities were discovered near the decoding center and the peptidyl transferase center, respectively. These results provide a structural basis for studying translation in Mtb as well as developing new tuberculosis drugs.

PubMed: 28977617
DOI: 10.1093/nar/gkx785

実験手法

ELECTRON MICROSCOPY (3.7 Å)