5V6M

Crystal Structure of Rabbit Anti-HIV-1 gp120 V3 Fab 10A3 in complex with V3 peptide ConB

5V6M の概要

• エントリーDOI: 10.2210/pdb5v6m/pdb
• 関連するPDBエントリー: 5V5L
• 分子名称: Light chain of Fab fragment of rabbit anti-HIV1 gp120 V3 mAb 10A3, Heavy chain of Fab fragment of rabbit anti-HIV1 gp120 V3 mAb 10A3, Envelope glycoprotein gp120 V3 peptide of Con B sequence, ... (5 entities in total)
• 機能のキーワード: hiv, gp120, v3, antibody, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 47401.87

構造登録者

Pan, R.,Kong, X.-P. (登録日: 2017-03-17, 公開日: 2018-01-17, 最終更新日: 2024-11-06)

主引用文献

Pan, R.,Qin, Y.,Banasik, M.,Lees, W.,Shepherd, A.J.,Cho, M.W.,Kong, X.P.
Increased epitope complexity correlated with antibody affinity maturation and a novel binding mode revealed by structures of rabbit antibodies against the third variable loop (V3) of HIV-1 gp120.
J. Virol., 2018

PubMed Abstract: The third variable (V3) loop of HIV-1 gp120 is an immunodominant region targeted by neutralizing antibodies (nAbs). Despite limited breadth, better characterization of the structural details of the interactions between these nAbs and their target epitopes would enhance our understanding of the mechanism of neutralization and facilitate designing better immunogens to induce nAbs with greater breadth. Recently, we isolated two anti-V3 neutralizing monoclonal antibodies (MAbs), 10A3 and 10A37, from a rabbit immunized with gp120 of the M group consensus sequence. In this study, crystal structures of these MAbs bound to target epitopes were determined. 10A3 binds to the V3 crown (TRKSIHIGPGRAF) using the cradle binding mode, similar to human V3 MAbs encoded by IGHV5-51 germ line genes, and its epitope structure resembles that bound to the human antibodies. In contrast, 10A37, which exhibits greater breadth and potency than 10A3, binds the V3 crown and the succeeding stem region (HIGPGRAFYTTGEI). Unexpectedly, the RAFYTT portion of the epitope existed as helical turns, a V3 structure that has not been observed previously. Its main chain-dominated antigen-antibody interactions not only explain the broad neutralization of 10A37 but also show that its epitope is a potential vaccine target to be further evaluated. In conclusion, our study provides novel insights about neutralization-susceptible epitope structures of the V3 loop of HIV-1 gp120 and demonstrates that, despite low amino acid sequence similarity to human antibody germ line genes, rabbits can serve as a useful animal model to evaluate human vaccine candidates. The apex crown of V3 of HIV-1 gp120 is the most immunogenic region of the surface glycoprotein, and many MAbs targeting this region have been developed. Structural understanding of V3 crown MAbs not only can help understand how antibody responses target this unique region but also contribute to immunogen design for vaccine development. We present here crystal structures of two neutralizing V3 MAbs, 10A3 and 10A37, developed from a rabbit immunized with gp120. Our analysis of 10A3 in complex with V3 provided a detailed example of how epitope complexity can evolve with affinity maturation, while that of 10A37 revealed a novel V3 binding mode targeting the C-terminal side of the V3 crown and showed that this region can form a helical structure. Our study provides novel insights about neutralization-susceptible V3 epitope structures and demonstrates that rabbits can serve as a useful animal model to evaluate human vaccine candidates.

PubMed: 29343576
DOI: 10.1128/JVI.01894-17

実験手法

X-RAY DIFFRACTION (1.9 Å)