5V52

Structure of TIGIT bound to nectin-2 (CD112)

5V52 の概要

• エントリーDOI: 10.2210/pdb5v52/pdb
• 分子名称: T-cell immunoreceptor with Ig and ITIM domains, Nectin-2, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: immune receptor, adhesion molecule, immunoglobulin fold, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 54385.84

構造登録者

Deuss, F.A.,Gully, B.S.,Rossjohn, J.,Berry, R. (登録日: 2017-03-13, 公開日: 2017-05-24, 最終更新日: 2024-10-30)

主引用文献

Deuss, F.A.,Gully, B.S.,Rossjohn, J.,Berry, R.
Recognition of nectin-2 by the natural killer cell receptor T cell immunoglobulin and ITIM domain (TIGIT).
J. Biol. Chem., 292:11413-11422, 2017

PubMed Abstract: T cell immunoglobulin and ITIM domain (TIGIT) is an inhibitory receptor expressed on the surface of natural killer (NK) cells. TIGIT recognizes nectin and nectin-like adhesion molecules and thus plays a critical role in the innate immune response to malignant transformation. Although the TIGIT nectin-like protein-5 (necl-5) interaction is well understood, how TIGIT engages nectin-2, a receptor that is broadly over-expressed in breast and ovarian cancer, remains unknown. Here, we show that TIGIT bound to the immunoglobulin domain of nectin-2 that is most distal from the membrane with an affinity of 6 μm, which was moderately lower than the affinity observed for the TIGIT/necl-5 interaction (3.2 μm). The TIGIT/nectin-2 binding disrupted pre-assembled nectin-2 oligomers, suggesting that receptor-ligand and ligand-ligand associations are mutually exclusive events. Indeed, the crystal structure of TIGIT bound to the first immunoglobulin domain of nectin-2 indicated that the receptor and ligand dock using the same molecular surface and a conserved "lock and key" binding motifs previously observed to mediate nectin/nectin homotypic interactions as well as TIGIT/necl-5 recognition. Using a mutagenesis approach, we dissected the energetic basis for the TIGIT/nectin-2 interaction and revealed that an "aromatic key" of nectin-2 is critical for this interaction, whereas variations in the lock were tolerated. Moreover, we found that the C-C' loop of the ligand dictates the TIGIT binding hierarchy. Altogether, these findings broaden our understanding of nectin/nectin receptor interactions and have implications for better understanding the molecular basis for autoimmune disease and cancer.

PubMed: 28515320
DOI: 10.1074/jbc.M117.786483

実験手法

X-RAY DIFFRACTION (3.1 Å)