5UZN

Crystal structure of Glorund qRRM3 domain

Summary for 5UZN

• Entry DOI: 10.2210/pdb5uzn/pdb
• Related: 5UZG 5UZM
• Descriptor: AT27789p, GLYCEROL, SULFATE ION, ... (4 entities in total)
• Functional Keywords: quasi-rna recognition motif, qrrm, rna binding protein
• Biological source: Drosophila melanogaster (Fruit fly)
• Total number of polymer chains: 1
• Total formula weight: 11154.35

Authors

Teramoto, T.,Hall, T.M.T. (deposition date: 2017-02-27, release date: 2017-03-29, Last modification date: 2023-10-04)

Primary citation

Tamayo, J.V.,Teramoto, T.,Chatterjee, S.,Hall, T.M.,Gavis, E.R.
The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition.
Cell Rep, 19:150-161, 2017

PubMed Abstract: The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.

PubMed: 28380354
DOI: 10.1016/j.celrep.2017.03.022

Experimental method

X-RAY DIFFRACTION (1.99 Å)