5UZM

Crystal structure of Glorund qRRM2 domain

5UZM の概要

• エントリーDOI: 10.2210/pdb5uzm/pdb
• 関連するPDBエントリー: 5UZG 5UZN
• 分子名称: AT27789p (2 entities in total)
• 機能のキーワード: quasi-rna recognition motif, qrrm, rna binding protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22048.80

構造登録者

Teramoto, T.,Hall, T.M.T. (登録日: 2017-02-27, 公開日: 2017-03-22, 最終更新日: 2023-10-04)

主引用文献

Tamayo, J.V.,Teramoto, T.,Chatterjee, S.,Hall, T.M.,Gavis, E.R.
The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition.
Cell Rep, 19:150-161, 2017

PubMed Abstract: The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.

PubMed: 28380354
DOI: 10.1016/j.celrep.2017.03.022

実験手法

X-RAY DIFFRACTION (1.552 Å)