5UZM
Crystal structure of Glorund qRRM2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-24 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.770, 28.969, 69.520 |
Unit cell angles | 90.00, 106.77, 90.00 |
Refinement procedure
Resolution | 32.542 - 1.552 |
R-factor | 0.1957 |
Rwork | 0.193 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2kg0 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.070 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.550 |
Rmerge | 0.050 |
Number of reflections | 23996 |
<I/σ(I)> | 34.6 |
Completeness [%] | 93.4 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M ammonium acetate, 20% v/v PEG 3350 |