5UZA

Adenine riboswitch aptamer domain labelled with iodo-uridine by position-selective labelling of RNA (PLOR)

Summary for 5UZA

• Entry DOI: 10.2210/pdb5uza/pdb
• Descriptor: RNA (71-MER), ADENINE, MAGNESIUM ION, ... (4 entities in total)
• Functional Keywords: rna, adenine riboswitch, iodo-uridine
• Biological source: Vibrio vulnificus
• Total number of polymer chains: 1
• Total formula weight: 23147.22

Authors

Liu, Y.,Stagno, J.R.,Wang, Y.-X. (deposition date: 2017-02-25, release date: 2018-02-28, Last modification date: 2024-03-06)

Primary citation

Liu, Y.,Holmstrom, E.,Yu, P.,Tan, K.,Zuo, X.,Nesbitt, D.J.,Sousa, R.,Stagno, J.R.,Wang, Y.X.
Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA.
Nat Protoc, 13:987-1005, 2018

PubMed Abstract: Site-specific incorporation of labeled nucleotides is an extremely useful synthetic tool for many structural studies (e.g., NMR, electron paramagnetic resonance (EPR), fluorescence resonance energy transfer (FRET), and X-ray crystallography) of RNA. However, specific-position-labeled RNAs >60 nt are not commercially available on a milligram scale. Position-selective labeling of RNA (PLOR) has been applied to prepare large RNAs labeled at desired positions, and all the required reagents are commercially available. Here, we present a step-by-step protocol for the solid-liquid hybrid phase method PLOR to synthesize 71-nt RNA samples with three different modification applications, containing (i) a CN-labeled segment; (ii) discrete residues modified with Cy3, Cy5, or biotin; or (iii) two iodo-U residues. The flexible procedure enables a wide range of downstream biophysical analyses using precisely localized functionalized nucleotides. All three RNAs were obtained in <2 d, excluding time for preparing reagents and optimizing experimental conditions. With optimization, the protocol can be applied to other RNAs with various labeling schemes, such as ligation of segmentally labeled fragments.

PubMed: 29651055
DOI: 10.1038/nprot.2018.002

Experimental method

X-RAY DIFFRACTION (2.22 Å)