5UXZ

X-ray crystal structure of Halotag bound to the P9 benzothiadiazole fluorogenic ligand

Summary for 5UXZ

• Entry DOI: 10.2210/pdb5uxz/pdb
• Related: 5UY1
• Descriptor: Haloalkane dehalogenase, N~2~-{[7-(dimethylamino)-2,1,3-benzothiadiazol-4-yl]sulfonyl}-N-hexyl-N~2~-methylglycinamide, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: haloalkane dehalogenase, fluorogenic, halo, tag, solvatochromatic, hydrolase
• Biological source: Rhodococcus rhodochrous
• Total number of polymer chains: 2
• Total formula weight: 70789.67

Authors

Dunham, N.P.,Boal, A.K. (deposition date: 2017-02-23, release date: 2017-03-08, Last modification date: 2017-11-22)

• Primary citation: Liu, Y.,Miao, K.,Dunham, N.P.,Liu, H.,Fares, M.,Boal, A.K.,Li, X.,Zhang, X.; The Cation-pi Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification.; Biochemistry, 56:1585-1595, 2017; PubMed: 28221782; DOI: 10.1021/acs.biochem.7b00056

Experimental method

X-RAY DIFFRACTION (1.92 Å)