5UXZ
X-ray crystal structure of Halotag bound to the P9 benzothiadiazole fluorogenic ligand
Summary for 5UXZ
Entry DOI | 10.2210/pdb5uxz/pdb |
Related | 5UY1 |
Descriptor | Haloalkane dehalogenase, N~2~-{[7-(dimethylamino)-2,1,3-benzothiadiazol-4-yl]sulfonyl}-N-hexyl-N~2~-methylglycinamide, CHLORIDE ION, ... (5 entities in total) |
Functional Keywords | haloalkane dehalogenase, fluorogenic, halo, tag, solvatochromatic, hydrolase |
Biological source | Rhodococcus rhodochrous |
Total number of polymer chains | 2 |
Total formula weight | 70789.67 |
Authors | Dunham, N.P.,Boal, A.K. (deposition date: 2017-02-23, release date: 2017-03-08, Last modification date: 2017-11-22) |
Primary citation | Liu, Y.,Miao, K.,Dunham, N.P.,Liu, H.,Fares, M.,Boal, A.K.,Li, X.,Zhang, X. The Cation-pi Interaction Enables a Halo-Tag Fluorogenic Probe for Fast No-Wash Live Cell Imaging and Gel-Free Protein Quantification. Biochemistry, 56:1585-1595, 2017 Cited by PubMed: 28221782DOI: 10.1021/acs.biochem.7b00056 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.92 Å) |
Structure validation
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