5UTT

SrtA sortase from Actinomyces oris

Summary for 5UTT

• Entry DOI: 10.2210/pdb5utt/pdb
• Descriptor: Sortase, CHLORIDE ION (3 entities in total)
• Functional Keywords: structural genomics, center for structural genomics of infectious diseases, csgid, hydrolase
• Biological source: Actinomyces oris
• Total number of polymer chains: 4
• Total formula weight: 84134.54

Authors

Osipiuk, J.,Ma, X.,Ton-That, H.,Anderson, W.F.,Joachimiak, A.,Center for Structural Genomics of Infectious Diseases (CSGID) (deposition date: 2017-02-15, release date: 2017-03-01, Last modification date: 2023-10-04)

Primary citation

Chang, C.,Wu, C.,Osipiuk, J.,Siegel, S.D.,Zhu, S.,Liu, X.,Joachimiak, A.,Clubb, R.T.,Das, A.,Ton-That, H.
Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes.
Proc.Natl.Acad.Sci.USA, 116:18041-18049, 2019

PubMed Abstract: Assembly of pili on the gram-positive bacterial cell wall involves 2 conserved transpeptidase enzymes named sortases: One for polymerization of pilin subunits and another for anchoring pili to peptidoglycan. How this machine controls pilus length and whether pilus length is critical for cell-to-cell interactions remain unknown. We report here in , a key colonizer in the development of oral biofilms, that genetic disruption of its housekeeping sortase SrtA generates exceedingly long pili, catalyzed by its pilus-specific sortase SrtC2 that possesses both pilus polymerization and cell wall anchoring functions. Remarkably, the deficient mutant fails to mediate interspecies interactions, or coaggregation, even though the coaggregation factor CafA is present at the pilus tip. Increasing ectopic expression of in the mutant progressively shortens pilus length and restores coaggregation accordingly, while elevated levels of shaft pilins and SrtC2 produce long pili and block coaggregation by SrtA bacteria. With structural studies, we uncovered 2 key structural elements in SrtA that partake in recognition of pilin substrates and regulate pilus length by inducing the capture and transfer of pilus polymers to the cell wall. Evidently, coaggregation requires proper positioning of the tip adhesin CafA via modulation of pilus length by the housekeeping sortase SrtA.

PubMed: 31427528
DOI: 10.1073/pnas.1907733116

Experimental method

X-RAY DIFFRACTION (1.7 Å)