5UQ6
PIG PURPLE ACID PHOSPHATASE COMPLEXED WITH PHOSPHATE IN TWO COORDINATION MODES ALONG WITH A BRIDGING HYDROXIDE ION
Summary for 5UQ6
| Entry DOI | 10.2210/pdb5uq6/pdb |
| Descriptor | Tartrate-resistant acid phosphatase type 5, 2-acetamido-2-deoxy-beta-D-glucopyranose, FE (III) ION, ... (7 entities in total) |
| Functional Keywords | transition state, metallohydrolase, hydroxide., hydrolase |
| Biological source | Sus scrofa (Pig) |
| Total number of polymer chains | 1 |
| Total formula weight | 35621.93 |
| Authors | Selleck, C.,Guddat, L.,Schenk, G.,Clayton, D. (deposition date: 2017-02-06, release date: 2017-05-24, Last modification date: 2024-10-30) |
| Primary citation | Selleck, C.,Clayton, D.,Gahan, L.R.,Mitic, N.,McGeary, R.P.,Pedroso, M.M.,Guddat, L.W.,Schenk, G. Visualization of the Reaction Trajectory and Transition State in a Hydrolytic Reaction Catalyzed by a Metalloenzyme. Chemistry, 23:4778-4781, 2017 Cited by PubMed Abstract: Metallohydrolases are a vast family of enzymes that play crucial roles in numerous metabolic pathways. Several members have emerged as targets for chemotherapeutics. Knowledge about their reaction mechanisms and associated transition states greatly aids the design of potent and highly specific drug leads. By using a high-resolution crystal structure, we have probed the trajectory of the reaction catalyzed by purple acid phosphatase, an enzyme essential for the integrity of bone structure. In particular, the transition state is visualized, thus providing detailed structural information that may be exploited in the design of specific inhibitors for the development of new anti-osteoporotic chemotherapeutics. PubMed: 28261912DOI: 10.1002/chem.201700866 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.182 Å) |
Structure validation
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