5ULP

Structure of the NS5 methyltransferase from Zika bound to MS2042

5ULP の概要

• エントリーDOI: 10.2210/pdb5ulp/pdb
• 関連するPDBエントリー: 5KQR 5KQS
• 分子名称: MRNA cap 0-1 NS5-type methyltransferase, 5'-{[(3S)-3-amino-3-carboxypropyl][(4-fluorophenyl)methyl]amino}-5'-deoxyadenosine, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: zika, flavivirus, ns5, methyltransferase, ms2042, sam analog, hydrolase
• 由来する生物種: Zika virus (strain Mr 766) (ZIKV)
• 細胞内の位置: Virion membrane ; Multi-pass membrane protein : B3U3M3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 60840.92

構造登録者

Jain, R.,Aggarwal, A.K. (登録日: 2017-01-25, 公開日: 2017-05-17, 最終更新日: 2024-11-20)

主引用文献

Jain, R.,Butler, K.V.,Coloma, J.,Jin, J.,Aggarwal, A.K.
Development of a S-adenosylmethionine analog that intrudes the RNA-cap binding site of Zika methyltransferase.
Sci Rep, 7:1632-1632, 2017

PubMed Abstract: The Zika virus (ZIKV) has emerged as a major health hazard. We present here a high resolution structure (1.55 Å) of ZIKV NS5 methyltransferase bound to a novel S-adenosylmethionine (SAM) analog in which a 4-fluorophenyl moiety substitutes for the methyl group. We show that the 4-fluorophenyl moiety extends into a portion of the RNA binding tunnel that typically contains the adenosine 2'OH of the RNA-cap moiety. Together, the new SAM analog and the high-resolution crystal structure are a step towards the development of antivirals against ZIKV and other flaviviruses.

PubMed: 28487506
DOI: 10.1038/s41598-017-01756-7

実験手法

X-RAY DIFFRACTION (1.55 Å)