5UKG

Crystal Structure of the genetically encoded calcium indicator K-GECO

Summary for 5UKG

• Entry DOI: 10.2210/pdb5ukg/pdb
• Descriptor: K-GECO, CALCIUM ION (3 entities in total)
• Functional Keywords: calcium indicator fluorescent protein, fluorescent protein
• Biological source: Entacmaea quadricolor
• Total number of polymer chains: 2
• Total formula weight: 93647.61

Authors

Schreiter, E.R. (deposition date: 2017-01-22, release date: 2018-02-07, Last modification date: 2025-04-02)

Primary citation

Shen, Y.,Dana, H.,Abdelfattah, A.S.,Patel, R.,Shea, J.,Molina, R.S.,Rawal, B.,Rancic, V.,Chang, Y.F.,Wu, L.,Chen, Y.,Qian, Y.,Wiens, M.D.,Hambleton, N.,Ballanyi, K.,Hughes, T.E.,Drobizhev, M.,Kim, D.S.,Koyama, M.,Schreiter, E.R.,Campbell, R.E.
A genetically encoded Ca2+indicator based on circularly permutated sea anemone red fluorescent protein eqFP578.
BMC Biol., 16:9-9, 2018

PubMed Abstract: Genetically encoded calcium ion (Ca) indicators (GECIs) are indispensable tools for measuring Ca dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores.

PubMed: 29338710
DOI: 10.1186/s12915-018-0480-0

Experimental method

X-RAY DIFFRACTION (2.36 Å)