5UHY
A Human Antibody Against Zika Virus Crosslinks the E Protein to Prevent Infection
Summary for 5UHY
| Entry DOI | 10.2210/pdb5uhy/pdb |
| EMDB information | 8548 |
| Descriptor | ZV67 Fab chain 1, ZV67 Fab chain 2, envelope protein (3 entities in total) |
| Functional Keywords | flavivirus, zika, human antibody, therapeutic, virus-immune system complex, virus/immune system |
| Biological source | Homo sapiens (Human) More |
| Total number of polymer chains | 7 |
| Total formula weight | 222837.55 |
| Authors | Hasan, S.S.,Miller, A.,Sapparapu, G.,Fernandez, E.,Klose, T.,Long, F.,Fokine, A.,Porta, J.C.,Jiang, W.,Diamond, M.S.,Crowe Jr., J.E.,Kuhn, R.J.,Rossmann, M.G. (deposition date: 2017-01-12, release date: 2017-03-29, Last modification date: 2024-11-13) |
| Primary citation | Hasan, S.S.,Miller, A.,Sapparapu, G.,Fernandez, E.,Klose, T.,Long, F.,Fokine, A.,Porta, J.C.,Jiang, W.,Diamond, M.S.,Crowe, J.E.,Kuhn, R.J.,Rossmann, M.G. A human antibody against Zika virus crosslinks the E protein to prevent infection. Nat Commun, 8:14722-14722, 2017 Cited by PubMed Abstract: The recent Zika virus (ZIKV) epidemic has been linked to unusual and severe clinical manifestations including microcephaly in fetuses of infected pregnant women and Guillian-Barré syndrome in adults. Neutralizing antibodies present a possible therapeutic approach to prevent and control ZIKV infection. Here we present a 6.2 Å resolution three-dimensional cryo-electron microscopy (cryoEM) structure of an infectious ZIKV (strain H/PF/2013, French Polynesia) in complex with the Fab fragment of a highly therapeutic and neutralizing human monoclonal antibody, ZIKV-117. The antibody had been shown to prevent fetal infection and demise in mice. The structure shows that ZIKV-117 Fabs cross-link the monomers within the surface E glycoprotein dimers as well as between neighbouring dimers, thus preventing the reorganization of E protein monomers into fusogenic trimers in the acidic environment of endosomes. PubMed: 28300075DOI: 10.1038/ncomms14722 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (6.2 Å) |
Structure validation
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