5U9F
3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II)
This is a non-PDB format compatible entry.
Summary for 5U9F
| Entry DOI | 10.2210/pdb5u9f/pdb |
| Related | 5U9G |
| EMDB information | 8521 8522 |
| Descriptor | 16S ribosomal RNA, 50S ribosomal protein L5, 50S ribosomal protein L6, ... (61 entities in total) |
| Functional Keywords | arfa rf2 ribosome rescue complex, ribosome |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 60 |
| Total formula weight | 2305499.01 |
| Authors | Demo, G.,Svidritskiy, E.,Madireddy, R.,Diaz-Avalos, R.,Grant, T.,Grigorieff, N.,Sousa, D.,Korostelev, A.A. (deposition date: 2016-12-16, release date: 2017-03-22, Last modification date: 2024-03-13) |
| Primary citation | Demo, G.,Svidritskiy, E.,Madireddy, R.,Diaz-Avalos, R.,Grant, T.,Grigorieff, N.,Sousa, D.,Korostelev, A.A. Mechanism of ribosome rescue by ArfA and RF2. Elife, 6:-, 2017 Cited by PubMed Abstract: ArfA rescues ribosomes stalled on truncated mRNAs by recruiting release factor RF2, which normally binds stop codons to catalyze peptide release. We report two 3.2 Å resolution cryo-EM structures - determined from a single sample - of the 70S ribosome with ArfA•RF2 in the A site. In both states, the ArfA C-terminus occupies the mRNA tunnel downstream of the A site. One state contains a compact inactive RF2 conformation. Ordering of the ArfA N-terminus in the second state rearranges RF2 into an extended conformation that docks the catalytic GGQ motif into the peptidyl-transferase center. Our work thus reveals the structural dynamics of ribosome rescue. The structures demonstrate how ArfA 'senses' the vacant mRNA tunnel and activates RF2 to mediate peptide release without a stop codon, allowing stalled ribosomes to be recycled. PubMed: 28300532DOI: 10.7554/eLife.23687 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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