5U9F

3.2 A cryo-EM ArfA-RF2 ribosome rescue complex (Structure II)

これはPDB形式変換不可エントリーです。

5U9F の概要

• エントリーDOI: 10.2210/pdb5u9f/pdb
• 関連するPDBエントリー: 5U9G
• EMDBエントリー: 8521 8522
• 分子名称: 16S ribosomal RNA, 50S ribosomal protein L5, 50S ribosomal protein L6, ... (61 entities in total)
• 機能のキーワード: arfa rf2 ribosome rescue complex, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2305499.01

構造登録者

Demo, G.,Svidritskiy, E.,Madireddy, R.,Diaz-Avalos, R.,Grant, T.,Grigorieff, N.,Sousa, D.,Korostelev, A.A. (登録日: 2016-12-16, 公開日: 2017-03-22, 最終更新日: 2024-03-13)

主引用文献

Demo, G.,Svidritskiy, E.,Madireddy, R.,Diaz-Avalos, R.,Grant, T.,Grigorieff, N.,Sousa, D.,Korostelev, A.A.
Mechanism of ribosome rescue by ArfA and RF2.
Elife, 6:-, 2017

PubMed Abstract: ArfA rescues ribosomes stalled on truncated mRNAs by recruiting release factor RF2, which normally binds stop codons to catalyze peptide release. We report two 3.2 Å resolution cryo-EM structures - determined from a single sample - of the 70S ribosome with ArfA•RF2 in the A site. In both states, the ArfA C-terminus occupies the mRNA tunnel downstream of the A site. One state contains a compact inactive RF2 conformation. Ordering of the ArfA N-terminus in the second state rearranges RF2 into an extended conformation that docks the catalytic GGQ motif into the peptidyl-transferase center. Our work thus reveals the structural dynamics of ribosome rescue. The structures demonstrate how ArfA 'senses' the vacant mRNA tunnel and activates RF2 to mediate peptide release without a stop codon, allowing stalled ribosomes to be recycled.

PubMed: 28300532
DOI: 10.7554/eLife.23687

実験手法

ELECTRON MICROSCOPY (3.2 Å)