5U76

Chicken Slo2.2 in a closed conformation vitrified in the presence of 300 mM NaCl

5U76 の概要

• エントリーDOI: 10.2210/pdb5u76/pdb
• 関連するPDBエントリー: 5U70
• EMDBエントリー: 8515 8517
• 分子名称: Potassium channel subfamily T member 1 (1 entity in total)
• 機能のキーワード: ion channel, potassium channel, transport protein
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 137409.81

構造登録者

Hite, R.K.,MacKinnon, R. (登録日: 2016-12-11, 公開日: 2017-02-08, 最終更新日: 2024-03-13)

主引用文献

Hite, R.K.,MacKinnon, R.
Structural Titration of Slo2.2, a Na(+)-Dependent K(+) Channel.
Cell, 168:390-399.e11, 2017

PubMed Abstract: The stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na-activated K channel, as a function of the Na concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na-dependent manner, without evidence of Na-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions.

PubMed: 28111072
DOI: 10.1016/j.cell.2016.12.030

実験手法

ELECTRON MICROSCOPY (3.76 Å)