5U70
Chicken Slo2.2 in an open conformation vitrified in the presence of 300 mM NaCl
Summary for 5U70
| Entry DOI | 10.2210/pdb5u70/pdb |
| Related | 5U76 |
| EMDB information | 8515 8517 |
| Descriptor | Potassium channel subfamily T member 1 (1 entity in total) |
| Functional Keywords | ion channel, potassium channel, transport protein |
| Biological source | Gallus gallus (Chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 137409.81 |
| Authors | Hite, R.K.,MacKinnon, R. (deposition date: 2016-12-09, release date: 2017-02-08, Last modification date: 2024-03-13) |
| Primary citation | Hite, R.K.,MacKinnon, R. Structural Titration of Slo2.2, a Na(+)-Dependent K(+) Channel. Cell, 168:390-399.e11, 2017 Cited by PubMed Abstract: The stable structural conformations that occur along the complete reaction coordinate for ion channel opening have never been observed. In this study, we describe the equilibrium ensemble of structures of Slo2.2, a neuronal Na-activated K channel, as a function of the Na concentration. We find that Slo2.2 exists in multiple closed conformations whose relative occupancies are independent of Na concentration. An open conformation emerges from an ensemble of closed conformations in a highly Na-dependent manner, without evidence of Na-dependent intermediates. In other words, channel opening is a highly concerted, switch-like process. The midpoint of the structural titration matches that of the functional titration. A maximum open conformation probability approaching 1.0 and maximum functional open probability approaching 0.7 imply that, within the class of open channels, there is a subclass that is not permeable to ions. PubMed: 28111072DOI: 10.1016/j.cell.2016.12.030 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.76 Å) |
Structure validation
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