5U3M
Crystal Structure of DH511.11P Fab in Complex with HIV-1 gp41 MPER Peptide
Summary for 5U3M
| Entry DOI | 10.2210/pdb5u3m/pdb |
| Related | 5U3J 5U3K 5U3L 5U3N 5U3O 5U3P |
| Descriptor | DH511.11P Fab Heavy Chain, DH511.11P Fab Light Chain, gp41 MPER peptide, ... (4 entities in total) |
| Functional Keywords | hiv-1, neutralizing, antibody, gp41, mper, immune system-viral protein complex, immune system/viral protein |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 3 |
| Total formula weight | 52825.50 |
| Authors | Ofek, G.,Wu, L.,Lougheed, C.S.,Williams, L.D.,Nicely, N.I.,Haynes, B.F. (deposition date: 2016-12-02, release date: 2017-02-15, Last modification date: 2024-10-30) |
| Primary citation | Williams, L.D.,Ofek, G.,Schatzle, S.,McDaniel, J.R.,Lu, X.,Nicely, N.I.,Wu, L.,Lougheed, C.S.,Bradley, T.,Louder, M.K.,McKee, K.,Bailer, R.T.,O'Dell, S.,Georgiev, I.S.,Seaman, M.S.,Parks, R.J.,Marshall, D.J.,Anasti, K.,Yang, G.,Nie, X.,Tumba, N.L.,Wiehe, K.,Wagh, K.,Korber, B.,Kepler, T.B.,Munir Alam, S.,Morris, L.,Kamanga, G.,Cohen, M.S.,Bonsignori, M.,Xia, S.M.,Montefiori, D.C.,Kelsoe, G.,Gao, F.,Mascola, J.R.,Moody, M.A.,Saunders, K.O.,Liao, H.X.,Tomaras, G.D.,Georgiou, G.,Haynes, B.F. Potent and broad HIV-neutralizing antibodies in memory B cells and plasma. Sci Immunol, 2:-, 2017 Cited by PubMed Abstract: Induction of broadly neutralizing antibodies (bnAbs) is a goal of HIV-1 vaccine development. Antibody 10E8, reactive with the distal portion of the membrane-proximal external region (MPER) of HIV-1 gp41, is broadly neutralizing. However, the ontogeny of distal MPER antibodies and the relationship of memory B cell to plasma bnAbs are poorly understood. HIV-1-specific memory B cell flow sorting and proteomic identification of anti-MPER plasma antibodies from an HIV-1-infected individual were used to isolate broadly neutralizing distal MPER bnAbs of the same B cell clonal lineage. Structural analysis demonstrated that antibodies from memory B cells and plasma recognized the envelope gp41 bnAb epitope in a distinct orientation compared with other distal MPER bnAbs. The unmutated common ancestor of this distal MPER bnAb was autoreactive, suggesting lineage immune tolerance control. Construction of chimeric antibodies of memory B cell and plasma antibodies yielded a bnAb that potently neutralized most HIV-1 strains. PubMed: 28783671DOI: 10.1126/sciimmunol.aal2200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.418 Å) |
Structure validation
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