5U1C

Structure of tetrameric HIV-1 Strand Transfer Complex Intasome

5U1C の概要

• エントリーDOI: 10.2210/pdb5u1c/pdb
• EMDBエントリー: 8481 8483
• 分子名称: HIV-1 Integrase, Sso7d chimera, DNA (11-MER), DNA (23-MER), ... (6 entities in total)
• 機能のキーワード: integrase, integration, transposase, transesterification, viral protein
• 由来する生物種: Sulfolobus solfataricus; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 213018.72

構造登録者

Lyumkis, D.,Passos, D. (登録日: 2016-11-28, 公開日: 2017-01-11, 最終更新日: 2024-03-13)

主引用文献

Passos, D.O.,Li, M.,Yang, R.,Rebensburg, S.V.,Ghirlando, R.,Jeon, Y.,Shkriabai, N.,Kvaratskhelia, M.,Craigie, R.,Lyumkis, D.
Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome.
Science, 355:89-92, 2017

PubMed Abstract: Like all retroviruses, HIV-1 irreversibly inserts a viral DNA (vDNA) copy of its RNA genome into host target DNA (tDNA). The intasome, a higher-order nucleoprotein complex composed of viral integrase (IN) and the ends of linear vDNA, mediates integration. Productive integration into host chromatin results in the formation of the strand transfer complex (STC) containing catalytically joined vDNA and tDNA. HIV-1 intasomes have been refractory to high-resolution structural studies. We used a soluble IN fusion protein to facilitate structural studies, through which we present a high-resolution cryo-electron microscopy (cryo-EM) structure of the core tetrameric HIV-1 STC and a higher-order form that adopts carboxyl-terminal domain rearrangements. The distinct STC structures highlight how HIV-1 can use the common retroviral intasome core architecture to accommodate different IN domain modules for assembly.

PubMed: 28059769
DOI: 10.1126/science.aah5163

実験手法

ELECTRON MICROSCOPY (3.9 Å)