5U04

Crystal structure of Zika virus NS5 RNA-dependent RNA polymerase

「5TIT」から置き換えられました

5U04 の概要

• エントリーDOI: 10.2210/pdb5u04/pdb
• 分子名称: Zika Virus NS5 RdRp, ZINC ION, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: ns5, zika, virus, rna, polymerase, drug, viral protein
• 由来する生物種: Zika virus (strain Mr 766) (ZIKV)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69245.41

構造登録者

Godoy, A.S.,Lima, G.M.A.,Oliveira, K.I.Z.,Torres, N.U.,Maluf, F.V.,Guido, R.V.C.,Oliva, G. (登録日: 2016-11-22, 公開日: 2017-02-22, 最終更新日: 2023-10-04)

主引用文献

Godoy, A.S.,Lima, G.M.,Oliveira, K.I.,Torres, N.U.,Maluf, F.V.,Guido, R.V.,Oliva, G.
Crystal structure of Zika virus NS5 RNA-dependent RNA polymerase.
Nat Commun, 8:14764-14764, 2017

PubMed Abstract: The current Zika virus (ZIKV) outbreak became a global health threat of complex epidemiology and devastating neurological impacts, therefore requiring urgent efforts towards the development of novel efficacious and safe antiviral drugs. Due to its central role in RNA viral replication, the non-structural protein 5 (NS5) RNA-dependent RNA-polymerase (RdRp) is a prime target for drug discovery. Here we describe the crystal structure of the recombinant ZIKV NS5 RdRp domain at 1.9 Å resolution as a platform for structure-based drug design strategy. The overall structure is similar to other flaviviral homologues. However, the priming loop target site, which is suitable for non-nucleoside polymerase inhibitor design, shows significant differences in comparison with the dengue virus structures, including a tighter pocket and a modified local charge distribution.

PubMed: 28345596
DOI: 10.1038/ncomms14764

実験手法

X-RAY DIFFRACTION (1.9 Å)