5TW2

Structure of mouse CD1d with bound alpha-galactosylsphingamide JG168

Summary for 5TW2

• Entry DOI: 10.2210/pdb5tw2/pdb
• Related: 1Z5L 5TW5
• Descriptor: Antigen-presenting glycoprotein CD1d1, Beta-2-microglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: mhc fold, antigen-presentation, glycolipid, t cells, immune system-inhibitor complex, immune system/inhibitor
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 2
• Total formula weight: 45996.02

Authors

Zajonc, D.M.,Wang, J. (deposition date: 2016-11-11, release date: 2017-09-20, Last modification date: 2024-11-20)

Primary citation

Guillaume, J.,Wang, J.,Janssens, J.,Remesh, S.G.,Risseeuw, M.D.P.,Decruy, T.,Froeyen, M.,Elewaut, D.,Zajonc, D.M.,Calenbergh, S.V.
Galactosylsphingamides: new alpha-GalCer analogues to probe the F'-pocket of CD1d.
Sci Rep, 7:4276-4276, 2017

PubMed Abstract: Invariant Natural Killer T-cells (iNKT-cells) are an attractive target for immune response modulation, as upon CD1d-mediated stimulation with KRN7000, a synthetic α-galactosylceramide, they produce a vast amount of cytokines. Here we present a synthesis that allows swift modification of the phytosphingosine side chain by amidation of an advanced methyl ester precursor. The resulting KRN7000 derivatives, termed α-galactosylsphingamides, were evaluated for their capacity to stimulate iNKT-cells. While introduction of the amide-motif in the phytosphingosine chain is tolerated for CD1d binding and TCR recognition, the studied α-galactosylsphingamides showed compromised antigenic properties.

PubMed: 28655912
DOI: 10.1038/s41598-017-04461-7

Experimental method

X-RAY DIFFRACTION (1.75 Å)