Room temperature structure of an extradiol ring-cleaving dioxygenase from B.fuscum determined using serial femtosecond crystallography

Summary for 5TRX

DescriptorHomoprotocatechuate 2,3-dioxygenase, FE (II) ION, CHLORIDE ION, ... (5 entities in total)
Functional Keywordsdioxygenase, 2-his-1-carboxylate facial triad, oxygen activation, double-flow focusing injector, oxidoreductase
Biological sourceBrevibacterium fuscum
Total number of polymer chains4
Total molecular weight167426.55
Kovaleva, E.G.,Oberthuer, D.,Tolstikova, A.,Mariani, V. (deposition date: 2016-10-27, release date: 2017-03-22, Last modification date: 2018-02-14)
Primary citation
Oberthuer, D.,Knoska, J.,Wiedorn, M.O.,Beyerlein, K.R.,Bushnell, D.A.,Kovaleva, E.G.,Heymann, M.,Gumprecht, L.,Kirian, R.A.,Barty, A.,Mariani, V.,Tolstikova, A.,Adriano, L.,Awel, S.,Barthelmess, M.,Dorner, K.,Xavier, P.L.,Yefanov, O.,James, D.R.,Nelson, G.,Wang, D.,Calvey, G.,Chen, Y.,Schmidt, A.,Szczepek, M.,Frielingsdorf, S.,Lenz, O.,Snell, E.,Robinson, P.J.,Sarler, B.,Belsak, G.,Macek, M.,Wilde, F.,Aquila, A.,Boutet, S.,Liang, M.,Hunter, M.S.,Scheerer, P.,Lipscomb, J.D.,Weierstall, U.,Kornberg, R.D.,Spence, J.C.H.,Pollack, L.,Chapman, H.N.,Bajt, S.
Double-flow focused liquid injector for efficient serial femtosecond crystallography
Sci. Rep., 7:44628-, 2017
DOI: 10.1038/srep44628
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.23720.4%3.6%0.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5trx
no rotation
Molmil generated image of 5trx
rotated about x axis by 90°
Molmil generated image of 5trx
rotated about y axis by 90°