5TPY

Crystal structure of an exonuclease resistant RNA from Zika virus

Summary for 5TPY

• Entry DOI: 10.2210/pdb5tpy/pdb
• Descriptor: RNA (71-MER), MAGNESIUM ION, HEXANE-1,6-DIOL, ... (4 entities in total)
• Functional Keywords: zika rna exonuclease resistance, rna
• Biological source: Zika virus
• Total number of polymer chains: 1
• Total formula weight: 23160.25

Authors

Akiyama, B.M.,Laurence, H.M.,Massey, A.R.,Costantino, D.A.,Xie, X.,Yang, Y.,Shi, P.-Y.,Nix, J.C.,Beckham, J.D.,Kieft, J.S. (deposition date: 2016-10-21, release date: 2016-12-14, Last modification date: 2023-10-04)

Primary citation

Akiyama, B.M.,Laurence, H.M.,Massey, A.R.,Costantino, D.A.,Xie, X.,Yang, Y.,Shi, P.Y.,Nix, J.C.,Beckham, J.D.,Kieft, J.S.
Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease.
Science, 354:1148-1152, 2016

PubMed Abstract: The outbreak of Zika virus (ZIKV) and associated fetal microcephaly mandates efforts to understand the molecular processes of infection. Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to pathogenicity in fetal mice. These viruses make sfRNAs by co-opting a cellular exonuclease via structured RNAs called xrRNAs. We found that ZIKV-infected monkey and human epithelial cells, mouse neurons, and mosquito cells produce sfRNAs. The RNA structure that is responsible for ZIKV sfRNA production forms a complex fold that is likely found in many pathogenic flaviviruses. Mutations that disrupt the structure affect exonuclease resistance in vitro and sfRNA formation during infection. The complete ZIKV xrRNA structure clarifies the mechanism of exonuclease resistance and identifies features that may modulate function in diverse flaviviruses.

PubMed: 27934765
DOI: 10.1126/science.aah3963

Experimental method

X-RAY DIFFRACTION (2.805 Å)