5TNF

Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 19,20-EpDPE hydrolysis intermediate

Summary for 5TNF

Entry DOI	10.2210/pdb5tnf/pdb
Related	3KD2 4DMC
Descriptor	CFTR inhibitory factor, (4Z,7Z,10Z,13Z,16Z,19R,20R)-19,20-dihydroxydocosa-4,7,10,13,16-pentaenoic acid (3 entities in total)
Functional Keywords	epoxide hydrolase, hydroxyalkyl-enzyme intermediate, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
Biological source	Pseudomonas aeruginosa (strain UCBPP-PA14)
Total number of polymer chains	4
Total formula weight	138104.87
Authors	Hvorecny, K.L.,Madden, D.R. (deposition date: 2016-10-14, release date: 2017-10-11, Last modification date: 2023-10-04)
Primary citation	Hvorecny, K.L.,Bahl, C.D.,Kitamura, S.,Lee, K.S.S.,Hammock, B.D.,Morisseau, C.,Madden, D.R. Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase. Structure, 25:697-707.e4, 2017 Cited by PubMed: 28392259 DOI: 10.1016/j.str.2017.03.002 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.75 Å)

Structure validation