Ca2+ bound aplysia Slo1

> Summary

Summary for 5TJI

EMDB informationEMD-8414 EMD-8410
DescriptorHigh conductance calcium-activated potassium channel
Functional Keywordsion channel, k+ channel, ca2+ bound, high conductance, membrane protein
Biological sourceAplysia californica (California sea hare)
Total number of polymer chains1
Total molecular weight121057.01
MacKinnon, R.,Tao, X.,Hite, R.K. (deposition date: 2016-10-04, release date: 2016-12-28, Last modification date: 2017-01-18)
Primary citation
Hite, R.K.,Tao, X.,MacKinnon, R.
Structural basis for gating the high-conductance Ca(2+)-activated K(+) channel.
Nature, 541:52-57, 2017
PubMed: 27974801
DOI: 10.1038/nature20775
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliers10.2%0.7%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5tji
no rotation
Molmil generated image of 5tji
rotated about x axis by 90°
Molmil generated image of 5tji
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 5tji
no rotation
Molmil generated image of 5tji
rotated about x axis by 90°
Molmil generated image of 5tji
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (5tji.pdb1.gz [546.06 KB])

> Structural details


Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
AHigh conductance calcium-activated potassium channelpolymer1070120308.01
UniProt (Q5QJC5)
Pfam (PF00520)
Pfam (PF03493)
Aplysia californica (California sea hare)
(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoatenon-polymer749.01

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains1
Total molecular weight120308.0
Non-Polymers*Number of molecules1
Total molecular weight749.0
All*Total molecular weight121057.0
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY

Cell axes1.0001.0001.000
Cell angles90.0090.0090.00
SpacegroupP 1
Resolution limits147.00 - 3.80
the highest resolution shell value3.899 - 3.800
the highest resolution shell value0.747
RMSD bond length0.006
RMSD bond angle0.861

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details


Functional Information from GO Data

A0016021cellular_componentintegral component of membrane
A0060072molecular_functionlarge conductance calcium-activated potassium channel activity

Functional Information from PDB Data

site_idNumber of ResiduesDetails

Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
PGW_5tji_A_11011(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate binding site
ATRP264PGW: (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate


Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails

Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails

Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

> Downloads

PDBj@FacebookPDBj@TwitterwwPDBwwPDB FoundationEM DataBank

Copyright © 2013-2017 Protein Data Bank Japan