Summary for 5TIP
| Entry DOI | 10.2210/pdb5tip/pdb |
| Descriptor | Major capsid protein, 6-deoxy-2,3-di-O-methyl-alpha-L-mannopyranose-(1-2)-beta-L-rhamnopyranose-(1-4)-beta-D-xylopyranose-(1-4)-[alpha-D-mannopyranose-(1-3)-alpha-D-rhamnopyranose-(1-3)][alpha-D-galactopyranose-(1-2)]alpha-L-fucopyranose-(1-3)-[beta-D-xylopyranose-(1-4)]beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-alpha-D-rhamnopyranose-(1-3)-[alpha-D-galactopyranose-(1-2)][beta-D-xylopyranose-(1-4)]alpha-L-fucopyranose-(1-3)-[beta-D-xylopyranose-(1-4)]beta-D-glucopyranose, ... (9 entities in total) |
| Functional Keywords | viral protein |
| Biological source | Paramecium bursaria Chlorella virus 1 (PBCV-1) |
| Total number of polymer chains | 4 |
| Total formula weight | 208698.33 |
| Authors | Klose, T.,De Castro, C.,Speciale, I.,Molinaro, A.,Van Etten, J.L.,Rossmann, M.G. (deposition date: 2016-10-03, release date: 2017-10-18, Last modification date: 2024-11-13) |
| Primary citation | De Castro, C.,Klose, T.,Speciale, I.,Lanzetta, R.,Molinaro, A.,Van Etten, J.L.,Rossmann, M.G. Structure of the chlorovirus PBCV-1 major capsid glycoprotein determined by combining crystallographic and carbohydrate molecular modeling approaches. Proc. Natl. Acad. Sci. U.S.A., 115:E44-E52, 2018 Cited by PubMed Abstract: The glycans of the major capsid protein (Vp54) of chlorella virus (PBCV-1) were recently described and found to be unusual. This prompted a reexamination of the previously reported Vp54 X-ray structure. A detailed description of the complete glycoprotein was achieved by combining crystallographic data with molecular modeling. The crystallographic data identified most of the monosaccharides located close to the protein backbone, but failed to detect those further from the glycosylation sites. Molecular modeling complemented this model by adding the missing monosaccharides and examined the conformational preference of the whole molecule, alone or within the crystallographic environment. Thus, combining X-ray crystallography with carbohydrate molecular modeling resulted in determining the complete glycosylated structure of a glycoprotein. In this case, it is the chlorovirus PBCV-1 major capsid protein. PubMed: 29255015DOI: 10.1073/pnas.1613432115 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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