5TIP

The Structure of the Major Capsid protein of PBCV-1

「1M3Y」から置き換えられました

5TIP の概要

• エントリーDOI: 10.2210/pdb5tip/pdb
• 分子名称: Major capsid protein, 6-deoxy-2,3-di-O-methyl-alpha-L-mannopyranose-(1-2)-beta-L-rhamnopyranose-(1-4)-beta-D-xylopyranose-(1-4)-[alpha-D-mannopyranose-(1-3)-alpha-D-rhamnopyranose-(1-3)][alpha-D-galactopyranose-(1-2)]alpha-L-fucopyranose-(1-3)-[beta-D-xylopyranose-(1-4)]beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-alpha-D-rhamnopyranose-(1-3)-[alpha-D-galactopyranose-(1-2)][beta-D-xylopyranose-(1-4)]alpha-L-fucopyranose-(1-3)-[beta-D-xylopyranose-(1-4)]beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: viral protein
• 由来する生物種: Paramecium bursaria Chlorella virus 1 (PBCV-1)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 208698.33

構造登録者

Klose, T.,De Castro, C.,Speciale, I.,Molinaro, A.,Van Etten, J.L.,Rossmann, M.G. (登録日: 2016-10-03, 公開日: 2017-10-18, 最終更新日: 2024-11-13)

主引用文献

De Castro, C.,Klose, T.,Speciale, I.,Lanzetta, R.,Molinaro, A.,Van Etten, J.L.,Rossmann, M.G.
Structure of the chlorovirus PBCV-1 major capsid glycoprotein determined by combining crystallographic and carbohydrate molecular modeling approaches.
Proc. Natl. Acad. Sci. U.S.A., 115:E44-E52, 2018

PubMed Abstract: The glycans of the major capsid protein (Vp54) of chlorella virus (PBCV-1) were recently described and found to be unusual. This prompted a reexamination of the previously reported Vp54 X-ray structure. A detailed description of the complete glycoprotein was achieved by combining crystallographic data with molecular modeling. The crystallographic data identified most of the monosaccharides located close to the protein backbone, but failed to detect those further from the glycosylation sites. Molecular modeling complemented this model by adding the missing monosaccharides and examined the conformational preference of the whole molecule, alone or within the crystallographic environment. Thus, combining X-ray crystallography with carbohydrate molecular modeling resulted in determining the complete glycosylated structure of a glycoprotein. In this case, it is the chlorovirus PBCV-1 major capsid protein.

PubMed: 29255015
DOI: 10.1073/pnas.1613432115

実験手法

X-RAY DIFFRACTION (2 Å)