5THF

Crystal structure of H3 hemagglutinin with insertion of two amino acids in the 150-loop from the A/Hong Kong/1/1968 (H3N2) influenza virus

5THF の概要

• エントリーDOI: 10.2210/pdb5thf/pdb
• 分子名称: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: influenza virus, hemagglutinin, ha, h10n8 (2013), receptor specificity, viral protein
• 由来する生物種: Influenza A virus (strain A/Hong Kong/1/1968 H3N2); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 173652.10

構造登録者

Tzarum, N.,Wilson, I.A. (登録日: 2016-09-29, 公開日: 2017-04-05, 最終更新日: 2024-10-30)

主引用文献

Tzarum, N.,de Vries, R.P.,Peng, W.,Thompson, A.J.,Bouwman, K.M.,McBride, R.,Yu, W.,Zhu, X.,Verheije, M.H.,Paulson, J.C.,Wilson, I.A.
The 150-Loop Restricts the Host Specificity of Human H10N8 Influenza Virus.
Cell Rep, 19:235-245, 2017

PubMed Abstract: Adaptation of influenza A viruses to new hosts are rare events but are the basis for emergence of new influenza pandemics in the human population. Thus, understanding the processes involved in such events is critical for anticipating potential pandemic threats. In 2013, the first case of human infection by an avian H10N8 virus was reported, yet the H10 hemagglutinin (HA) maintains avian receptor specificity. However, the 150-loop of H10 HA, as well as related H7 and H15 subtypes, contains a two-residue insert that can potentially block human receptor binding. Mutation of the 150-loop on the background of Q226L and G228S mutations, which arose in the receptor-binding site of human pandemic H2 and H3 viruses, resulted in acquisition of human-type receptor specificity. Crystal structures of H10 HA mutants with human and avian receptor analogs, receptor-binding studies, and tissue staining experiments illustrate the important role of the 150-loop in H10 receptor specificity.

PubMed: 28402848
DOI: 10.1016/j.celrep.2017.03.054

実験手法

X-RAY DIFFRACTION (2.59 Å)