5TCQ

Near-atomic resolution cryo-EM structure of the Salmonella SPI-1 type III secretion injectisome secretin InvG

> Summary

Summary for 5TCQ

Related5TCP 5TCR
EMDB informationEMD-8399 EMD-8398 EMD-8400 EMD-8401
DescriptorProtein InvG
Functional Keywordsbacterial, secretion, injectisome, membrane protein
Biological sourceSalmonella enterica subsp. enterica serovar Typhimurium
Cellular locationCell outer membrane ; Peripheral membrane protein  P35672
Total number of polymer chains15
Total molecular weight927533.39
Authors
Worrall, L.J.,Hong, C.,Vuckovic, M.,Bergeron, J.R.C.,Huang, R.K.,Yu, Z.,Strynadka, N.C.J. (deposition date: 2016-09-15, release date: 2016-12-21, modification date: 2017-01-11)
Primary citation
Worrall, L.J.,Hong, C.,Vuckovic, M.,Deng, W.,Bergeron, J.R.,Majewski, D.D.,Huang, R.K.,Spreter, T.,Finlay, B.B.,Yu, Z.,Strynadka, N.C.
Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body.
Nature, 540:597-601, 2016
PubMed: 27974800
DOI: 10.1038/nature20576
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliers22.7%0MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5tcq
no rotation
Molmil generated image of 5tcq
rotated about x axis by 90°
Molmil generated image of 5tcq
rotated about y axis by 90°

> Structural details

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains15
Total molecular weight927533.4
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight927533.4
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:ELECTRON MICROSCOPY

Resolution limits -
the highest resolution shell value -

Data Collection Statistics

Resolution limits -
the highest resolution shell value -

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0009279cellular_componentcell outer membrane
A0008565molecular_functionprotein transporter activity
A0009405biological_processpathogenesis
A0009306biological_processprotein secretion
B0009279cellular_componentcell outer membrane
B0008565molecular_functionprotein transporter activity
B0009405biological_processpathogenesis
B0009306biological_processprotein secretion
C0009279cellular_componentcell outer membrane
C0008565molecular_functionprotein transporter activity
C0009405biological_processpathogenesis
C0009306biological_processprotein secretion
D0009279cellular_componentcell outer membrane
D0008565molecular_functionprotein transporter activity
D0009405biological_processpathogenesis
D0009306biological_processprotein secretion
E0009279cellular_componentcell outer membrane
E0008565molecular_functionprotein transporter activity
E0009405biological_processpathogenesis
E0009306biological_processprotein secretion
F0009279cellular_componentcell outer membrane
F0008565molecular_functionprotein transporter activity
F0009405biological_processpathogenesis
F0009306biological_processprotein secretion
G0009279cellular_componentcell outer membrane
G0008565molecular_functionprotein transporter activity
G0009405biological_processpathogenesis
G0009306biological_processprotein secretion
H0009279cellular_componentcell outer membrane
H0008565molecular_functionprotein transporter activity
H0009405biological_processpathogenesis
H0009306biological_processprotein secretion
I0009279cellular_componentcell outer membrane
I0008565molecular_functionprotein transporter activity
I0009405biological_processpathogenesis
I0009306biological_processprotein secretion
J0009279cellular_componentcell outer membrane
J0008565molecular_functionprotein transporter activity
J0009405biological_processpathogenesis
J0009306biological_processprotein secretion
K0009279cellular_componentcell outer membrane
K0008565molecular_functionprotein transporter activity
K0009405biological_processpathogenesis
K0009306biological_processprotein secretion
L0009279cellular_componentcell outer membrane
L0008565molecular_functionprotein transporter activity
L0009405biological_processpathogenesis
L0009306biological_processprotein secretion
M0009279cellular_componentcell outer membrane
M0008565molecular_functionprotein transporter activity
M0009405biological_processpathogenesis
M0009306biological_processprotein secretion
N0009279cellular_componentcell outer membrane
N0008565molecular_functionprotein transporter activity
N0009405biological_processpathogenesis
N0009306biological_processprotein secretion
O0009279cellular_componentcell outer membrane
O0008565molecular_functionprotein transporter activity
O0009405biological_processpathogenesis
O0009306biological_processprotein secretion
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

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Functional Information from SwissProt/UniProt

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Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

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