5T77
Crystal structure of the MOP flippase MurJ
Summary for 5T77
| Entry DOI | 10.2210/pdb5t77/pdb |
| Descriptor | Putative lipid II flippase MurJ, CHLORIDE ION, ZINC ION, ... (9 entities in total) |
| Functional Keywords | transporter, flippase, peptidoglycan, transport protein |
| Biological source | Thermosipho africanus (strain TCF52B) |
| Total number of polymer chains | 1 |
| Total formula weight | 69166.74 |
| Authors | Kuk, A.C.Y.,Lee, S.-Y. (deposition date: 2016-09-02, release date: 2016-12-28, Last modification date: 2024-03-06) |
| Primary citation | Kuk, A.C.,Mashalidis, E.H.,Lee, S.Y. Crystal structure of the MOP flippase MurJ in an inward-facing conformation. Nat. Struct. Mol. Biol., 24:171-176, 2017 Cited by PubMed Abstract: Peptidoglycan (PG) protects bacteria from osmotic lysis, and its biogenesis is a key antibiotic target. A central step in PG biosynthesis is flipping of the lipid-linked PG precursor lipid II across the cytoplasmic membrane for subsequent incorporation into PG. MurJ, part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) transporter superfamily, was recently shown to carry out this process. However, understanding of how MurJ flips lipid II, and of how MOP transporters operate in general, remains limited due to a lack of structural information. Here we present a crystal structure of MurJ from Thermosipho africanus in an inward-facing conformation at 2.0-Å resolution. A hydrophobic groove is formed by two C-terminal transmembrane helices, which leads into a large central cavity that is mostly cationic. Our studies not only provide the first structural glimpse of MurJ but also suggest that alternating access is important for MurJ function, which may be applicable to other MOP superfamily transporters. PubMed: 28024149DOI: 10.1038/nsmb.3346 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report
