5T6W

HLA-B*57:01 presenting SSTRGISQLW

5T6W の概要

• エントリーDOI: 10.2210/pdb5t6w/pdb
• 関連するPDBエントリー: 5T6X 5T6Y 5T6Z 5T70
• 分子名称: HLA class I histocompatibility antigen, B-57 alpha chain, Beta-2-microglobulin, Decapeptide: SER-SER-THR-ARG-GLY-ILE-SER-GLN-LEU-TRP, ... (5 entities in total)
• 機能のキーワード: human leukocyte antigen immunoglobulin domain antigen presentation, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P18465; Secreted . Note=(Microbial infection) In the presence of M: P61769
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 44711.68

構造登録者

Pymm, P.,Rossjohn, J.,Vivian, J.P. (登録日: 2016-09-01, 公開日: 2017-03-01, 最終更新日: 2024-10-16)

主引用文献

Pymm, P.,Illing, P.T.,Ramarathinam, S.H.,O'Connor, G.M.,Hughes, V.A.,Hitchen, C.,Price, D.A.,Ho, B.K.,McVicar, D.W.,Brooks, A.G.,Purcell, A.W.,Rossjohn, J.,Vivian, J.P.
MHC-I peptides get out of the groove and enable a novel mechanism of HIV-1 escape.
Nat. Struct. Mol. Biol., 24:387-394, 2017

PubMed Abstract: Major histocompatibility complex class I (MHC-I) molecules play a crucial role in immunity by capturing peptides for presentation to T cells and natural killer (NK) cells. The peptide termini are tethered within the MHC-I antigen-binding groove, but it is unknown whether other presentation modes occur. Here we show that 20% of the HLA-B*57:01 peptide repertoire comprises N-terminally extended sets characterized by a common motif at position 1 (P1) to P2. Structures of HLA-B*57:01 presenting N-terminally extended peptides, including the immunodominant HIV-1 Gag epitope TW10 (TSTLQEQIGW), showed that the N terminus protrudes from the peptide-binding groove. The common escape mutant TSNLQEQIGW bound HLA-B*57:01 canonically, adopting a dramatically different conformation than the TW10 peptide. This affected recognition by killer cell immunoglobulin-like receptor (KIR) 3DL1 expressed on NK cells. We thus define a previously uncharacterized feature of the human leukocyte antigen class I (HLA-I) immunopeptidome that has implications for viral immune escape. We further suggest that recognition of the HLA-B*57:01-TW10 epitope is governed by a 'molecular tension' between the adaptive and innate immune systems.

PubMed: 28218747
DOI: 10.1038/nsmb.3381

実験手法

X-RAY DIFFRACTION (1.9 Å)