5T5M

TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE FROM METHANOTHERMOBACTER WOLFEII, TRIGONAL FORM AT 2.5 A.

5T5M の概要

• エントリーDOI: 10.2210/pdb5t5m/pdb
• 分子名称: Tungsten formylmethanofuran dehydrogenase subunit fwdA, POTASSIUM ION, IRON/SULFUR CLUSTER, ... (15 entities in total)
• 機能のキーワード: co2 fixation, metallohydrolase, formate dehydrogenase, tungstopterin, methanogenesis, green house gas, methanothermobacter wolfeii, iron sulfur cluster, ferredoxin, beta helicoidal, channel, formate, co2, methanofuran, formylmethanofuran, nanomachine, binuclear center, tungsten, gate, coupling, enzyme, anaerobic, carboxylysine, oxidoreductase
• 由来する生物種: Methanothermobacter wolfeii; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 207509.91

構造登録者

Wagner, T.,Ermler, U.,Shima, S. (登録日: 2016-08-31, 公開日: 2016-10-19, 最終更新日: 2024-01-17)

主引用文献

Wagner, T.,Ermler, U.,Shima, S.
The methanogenic CO2 reducing-and-fixing enzyme is bifunctional and contains 46 [4Fe-4S] clusters.
Science, 354:114-117, 2016

PubMed Abstract: Biological methane formation starts with a challenging adenosine triphosphate (ATP)-independent carbon dioxide (CO) fixation process. We explored this enzymatic process by solving the x-ray crystal structure of formyl-methanofuran dehydrogenase, determined here as Fwd(ABCDFG) and Fwd(ABCDFG) complexes, from Methanothermobacter wolfeii The latter 800-kilodalton apparatus consists of four peripheral catalytic sections and an electron-supplying core with 46 electronically coupled [4Fe-4S] clusters. Catalysis is separately performed by subunits FwdBD (FwdB and FwdD), which are related to tungsten-containing formate dehydrogenase, and subunit FwdA, a binuclear metal center carrying amidohydrolase. CO is first reduced to formate in FwdBD, which then diffuses through a 43-angstrom-long tunnel to FwdA, where it condenses with methanofuran to formyl-methanofuran. The arrangement of [4Fe-4S] clusters functions as an electron relay but potentially also couples the four tungstopterin active sites over 206 angstroms.

PubMed: 27846502
DOI: 10.1126/science.aaf9284

実験手法

X-RAY DIFFRACTION (2.5 Å)