5T1J

Crystal Structure of the Tbox DNA binding domain of the transcription factor T-bet

5T1J の概要

• エントリーDOI: 10.2210/pdb5t1j/pdb
• 分子名称: T-box transcription factor TBX21, DNA (2 entities in total)
• 機能のキーワード: t-bet, tbox, tbx21, dna looping, transcriptional regulation, master regulator, transcription factor, dna binding, transcription-dna complex, transcription/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Nucleus: Q9JKD8
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 76372.23

構造登録者

Liu, C.F.,Brandt, G.S.,Hoang, Q.,Hwang, E.S.,Naumova, N.,Lazarevic, V.,Dekker, J.,Glimcher, L.H.,Ringe, D.,Petsko, G.A. (登録日: 2016-08-19, 公開日: 2016-10-26, 最終更新日: 2023-12-27)

主引用文献

Liu, C.F.,Brandt, G.S.,Hoang, Q.Q.,Naumova, N.,Lazarevic, V.,Hwang, E.S.,Dekker, J.,Glimcher, L.H.,Ringe, D.,Petsko, G.A.
Crystal structure of the DNA binding domain of the transcription factor T-bet suggests simultaneous recognition of distant genome sites.
Proc.Natl.Acad.Sci.USA, 113:E6572-E6581, 2016

PubMed Abstract: The transcription factor T-bet (Tbox protein expressed in T cells) is one of the master regulators of both the innate and adaptive immune responses. It plays a central role in T-cell lineage commitment, where it controls the T1 response, and in gene regulation in plasma B-cells and dendritic cells. T-bet is a member of the Tbox family of transcription factors; however, T-bet coordinately regulates the expression of many more genes than other Tbox proteins. A central unresolved question is how T-bet is able to simultaneously recognize distant Tbox binding sites, which may be located thousands of base pairs away. We have determined the crystal structure of the Tbox DNA binding domain (DBD) of T-bet in complex with a palindromic DNA. The structure shows a quaternary structure in which the T-bet dimer has its DNA binding regions splayed far apart, making it impossible for a single dimer to bind both sites of the DNA palindrome. In contrast to most other Tbox proteins, a single T-bet DBD dimer binds simultaneously to identical half-sites on two independent DNA. A fluorescence-based assay confirms that T-bet dimers are able to bring two independent DNA molecules into close juxtaposition. Furthermore, chromosome conformation capture assays confirm that T-bet functions in the direct formation of chromatin loops in vitro and in vivo. The data are consistent with a looping/synapsing model for transcriptional regulation by T-bet in which a single dimer of the transcription factor can recognize and coalesce distinct genetic elements, either a promoter plus a distant regulatory element, or promoters on two different genes.

PubMed: 27791029
DOI: 10.1073/pnas.1613914113

実験手法

X-RAY DIFFRACTION (2.947 Å)