5T1F
Crystal structure of Phaeospaeria nodrum fructosyl peptide oxidase mutant Asn56Ala
Summary for 5T1F
| Entry DOI | 10.2210/pdb5t1f/pdb |
| Related | 5T1E |
| Descriptor | Uncharacterized protein, FLAVIN-ADENINE DINUCLEOTIDE, ACETIC ACID, ... (4 entities in total) |
| Functional Keywords | fructosyl peptide oxidase, fad, oxidoreductase |
| Biological source | Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) |
| Total number of polymer chains | 1 |
| Total formula weight | 49903.12 |
| Authors | Yoshida, H.,Shimasaki, T.,Kamitori, S.,Sode, K. (deposition date: 2016-08-19, release date: 2017-06-28, Last modification date: 2024-11-13) |
| Primary citation | Shimasaki, T.,Yoshida, H.,Kamitori, S.,Sode, K. X-ray structures of fructosyl peptide oxidases revealing residues responsible for gating oxygen access in the oxidative half reaction Sci Rep, 7:2790-2790, 2017 Cited by PubMed Abstract: Current enzymatic systems for quantifying glycated hemoglobin are based on the FAD-containing enzyme fructosyl peptide oxidase (FPOX). FPOX has substrate specificity for fructosyl- N-valyl-histidine derived from proteolytic digestion of the N-terminus of the HbA1c β-chain. This study reports the X-ray structures of the wild-type and Asn56Ala (N56A) mutant of Phaeosphaeria nodorum fructosyl peptide oxidase (PnFPOX) to elucidate the residues responsible for the oxidative half-reaction. N56A showed decreased oxidase activity compared to the wild -type, while its dye-mediated dehydrogenase activity was higher than that of wild type. In wild-type PnFPOX, Asn56 forms a hydrogen bond with Lys274, thereby preventing it from forming a salt bridge with Asp54. By contrast, Lys274 of PnFPOX N56A moves toward Asp54, and they approach each other to form a salt bridge at a distance of 2.92-3.35 Å. Site-directed mutagenesis studies and protein channel analysis suggest that Asp54 assists in accepting oxygen properly at the position of the bound water molecule in the main oxygen channel. These results reveal that Asn56 in PnFPOX is essential for maintaining an effective oxygen accession path, and support the role of Asp54 as a gate keeper that cooperates with Lys274 to enable oxygen to reach the active site properly. PubMed: 28584265DOI: 10.1038/s41598-017-02657-5 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.98 Å) |
Structure validation
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