5SZI

Structure of human Rab8a in complex with the bMERB domain of Mical-cL

5SZI の概要

• エントリーDOI: 10.2210/pdb5szi/pdb
• 分子名称: Ras-related protein Rab-8A, MICAL C-terminal-like protein, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: mical-cl, duf3585, mical, rab effector, rab8a, transport protein, endocytosis
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cell membrane ; Lipid-anchor ; Cytoplasmic side : P61006; Cytoplasm : Q6ZW33
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 42913.63

構造登録者

Rai, A.,Oprisko, A.,Campos, J.,Fu, Y.,Friese, T.,Itzen, A.,Goody, R.S.,Mueller, M.P.,Gazdag, E.M. (登録日: 2016-08-14, 公開日: 2016-08-24, 最終更新日: 2024-01-17)

主引用文献

Rai, A.,Oprisko, A.,Campos, J.,Fu, Y.,Friese, T.,Itzen, A.,Goody, R.S.,Gazdag, E.M.,Muller, M.P.
bMERB domains are bivalent Rab8 family effectors evolved by gene duplication.
Elife, 5:-, 2016

PubMed Abstract: In their active GTP-bound form, Rab proteins interact with proteins termed effector molecules. In this study, we have thoroughly characterized a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. Within our study, we show that these effectors display a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate binding sites. Structural analysis allowed us to explain the specificity towards Rab8 family members and the presence of two similar Rab binding sites that must have evolved via gene duplication. This study is the first to thoroughly characterize a Rab effector protein that contains two separate Rab binding sites within a single domain, allowing Micals and EHBPs to bind two Rabs simultaneously, thus suggesting previously unknown functions of these effector molecules in endosomal trafficking.

PubMed: 27552051
DOI: 10.7554/eLife.18675

実験手法

X-RAY DIFFRACTION (2.85 Å)