Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

5OPW

Crystal structure of the GroEL mutant A109C

Summary for 5OPW
Entry DOI10.2210/pdb5opw/pdb
Related5OPX
Descriptor60 kDa chaperonin (1 entity in total)
Functional Keywordschaperonin, chaperone
Biological sourceEscherichia coli (strain K12)
Cellular locationCytoplasm : P0A6F5
Total number of polymer chains14
Total formula weight802095.92
Authors
Yan, X.,Shi, Q.,Bracher, A.,Milicic, G.,Singh, A.K.,Hartl, F.U.,Hayer-Hartl, M. (deposition date: 2017-08-10, release date: 2018-01-10, Last modification date: 2024-11-20)
Primary citationYan, X.,Shi, Q.,Bracher, A.,Milicic, G.,Singh, A.K.,Hartl, F.U.,Hayer-Hartl, M.
GroEL Ring Separation and Exchange in the Chaperonin Reaction.
Cell, 172:605-617.e11, 2018
Cited by
PubMed Abstract: The bacterial chaperonin GroEL and its cofactor, GroES, form a nano-cage for a single molecule of substrate protein (SP) to fold in isolation. GroEL and GroES undergo an ATP-regulated interaction cycle to close and open the folding cage. GroEL consists of two heptameric rings stacked back to back. Here, we show that GroEL undergoes transient ring separation, resulting in ring exchange between complexes. Ring separation occurs upon ATP-binding to the trans ring of the asymmetric GroEL:7ADP:GroES complex in the presence or absence of SP and is a consequence of inter-ring negative allostery. We find that a GroEL mutant unable to perform ring separation is folding active but populates symmetric GroEL:GroES complexes, where both GroEL rings function simultaneously rather than sequentially. As a consequence, SP binding and release from the folding chamber is inefficient, and E. coli growth is impaired. We suggest that transient ring separation is an integral part of the chaperonin mechanism.
PubMed: 29336887
DOI: 10.1016/j.cell.2017.12.010
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (3.19 Å)
Structure validation

227561

PDB entries from 2024-11-20

PDB statisticsPDBj update infoContact PDBjnumon