5OOM

Structure of a native assembly intermediate of the human mitochondrial ribosome with unfolded interfacial rRNA

Summary for 5OOM

EMDB information3843
Descriptor16S ribosomal RNA, mitochondrial tRNAVal, 39S ribosomal protein L2, mitochondrial, ... (58 entities in total)
Functional Keywordsribosome, mitochondria, biogenesis, translation, electron cryomicroscopy
Biological sourceHomo sapiens (Human)
Cellular locationMitochondrion  Q5T653 P09001 Q9BYD3 Q9BYD2 Q7Z7H8 Q9Y3B7 Q9BYD1 Q6P1L8 Q9P015 Q9NX20 Q9NRX2 Q9H0U6 P49406 Q9BYC9 Q7Z2W9 Q9NWU5 Q16540 Q96A35 Q9P0M9 Q13084 Q9HD33 Q8TCC3 Q9BYC8 O75394 Q9BQ48 Q9NZE8 Q9BZE1 Q96DV4 Q9NYK5 Q9NQ50 Q8IXM3 Q9Y6G3 Q8N983 Q9H9J2 Q9BRJ2 Q9H2W6 Q96GC5 Q13405 Q8N5N7 Q4U2R6 Q86TS9 Q96EL3 Q6P161 Q7Z7F7 Q9BQC6 Q14197 Q8TAE8 Q9NVS2 Q9NP92 O14561
Mitochondrion matrix  Q96EH3
Total number of polymer chains55
Total molecular weight1784460.89
Authors
Brown, A.,Rathore, S.,Kimanius, D.,Aibara, S.,Bai, X.C.,Rorbach, J.,Amunts, A.,Ramakrishnan, V. (deposition date: 2017-08-08, release date: 2017-09-13, Last modification date: 2018-01-31)
Primary citation
Brown, A.,Rathore, S.,Kimanius, D.,Aibara, S.,Bai, X.C.,Rorbach, J.,Amunts, A.,Ramakrishnan, V.
Structures of the human mitochondrial ribosome in native states of assembly.
Nat. Struct. Mol. Biol., 24:866-869, 2017
PubMed: 28892042 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb.3464
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone60.1%1.3%0.44MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5oom
no rotation
Molmil generated image of 5oom
rotated about x axis by 90°
Molmil generated image of 5oom
rotated about y axis by 90°