5OLN

X-Ray Structure of the Complex Pyrimidine-nucleoside phosphorylase from Bacillus subtilis at 1.88 A

5OLN の概要

• エントリーDOI: 10.2210/pdb5oln/pdb
• 関連するPDBエントリー: 5EP8
• 分子名称: Pyrimidine-nucleoside phosphorylase, IMIDAZOLE, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: transferase, pyrimidine-nucleoside phosphorylase, np-2 superfamily
• 由来する生物種: Bacillus subtilis (strain 168)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93031.83

構造登録者

Balaev, V.V.,Prokofev, I.I.,Gabdoulkhakov, A.G.,Betzel, C.,Lashkov, A.A. (登録日: 2017-07-28, 公開日: 2018-04-18, 最終更新日: 2024-01-17)

主引用文献

Balaev, V.V.,Prokofev, I.I.,Gabdoulkhakov, A.G.,Betzel, C.,Lashkov, A.A.
Crystal structure of pyrimidine-nucleoside phosphorylase from Bacillus subtilis in complex with imidazole and sulfate.
Acta Crystallogr F Struct Biol Commun, 74:193-197, 2018

PubMed Abstract: Pyrimidine-nucleoside phosphorylase catalyzes the phosphorolytic cleavage of thymidine and uridine with equal activity. Investigation of this protein is essential for anticancer drug design. Here, the structure of this protein from Bacillus subtilis in complex with imidazole and sulfate is reported at 1.9 Å resolution, which is an improvement on the previously reported structure at 2.6 Å resolution. The localization and position of imidazole in the nucleoside-binding site reflects the possible binding of ligands that possess an imidazole ring.

PubMed: 29633966
DOI: 10.1107/S2053230X18002935

実験手法

X-RAY DIFFRACTION (1.88 Å)