5OJX
Crystal structure of regulator protein 2 (PamR2) from the pamamycin biosynthetic gene cluster of Streptomyces alboniger
Summary for 5OJX
| Entry DOI | 10.2210/pdb5ojx/pdb |
| Descriptor | TetR family transcription regulator (2 entities in total) |
| Functional Keywords | transcription regulator, tetr family transcription regulator, streptomyces alboniger, regulator protein 2, pamamycin biosynthesis, transcription |
| Biological source | Streptomyces alboniger |
| Total number of polymer chains | 1 |
| Total formula weight | 28250.69 |
| Authors | Schmelz, S.,Rebets, Y.,Luzhetskyy, A.,Scrima, A. (deposition date: 2017-07-24, release date: 2018-04-11, Last modification date: 2024-01-17) |
| Primary citation | Rebets, Y.,Schmelz, S.,Gromyko, O.,Tistechok, S.,Petzke, L.,Scrima, A.,Luzhetskyy, A. Design, development and application of whole-cell based antibiotic-specific biosensor. Metab. Eng., 47:263-270, 2018 Cited by PubMed Abstract: Synthetic biology techniques hold great promise for optimising the production of natural products by microorganisms. However, evaluating the phenotype of a modified bacterium represents a major bottleneck to the engineering cycle - particularly for antibiotic-producing actinobacteria strains, which grow slowly and are challenging to genetically manipulate. Here, we report the generation and application of antibiotic-specific whole-cell biosensor derived from TetR transcriptional repressor for use in identifying and optimising antibiotic producers. The constructed biosensor was successfully used to improve production of polyketide antibiotic pamamycin. However, an initial biosensor based on native genetic elements had inadequate dynamic and operating ranges. To overcome these limitations, we fine-tuned biosensor performance through alterations of the promoter and operator of output module and the ligand affinity of transcription factor module, which enabled us to deduce recommendations for building and application of actinobacterial biosensors. PubMed: 29609044DOI: 10.1016/j.ymben.2018.03.019 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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