5OJJ

Crystal structure of the Zn-bound ubiquitin-conjugating enzyme Ube2T

5OJJ の概要

• エントリーDOI: 10.2210/pdb5ojj/pdb
• 分子名称: Ubiquitin-conjugating enzyme E2 T, ACETATE ION, ZINC ION, ... (5 entities in total)
• 機能のキーワード: ubiquitin conjugating enzyme, zinc, domain swap, oligomer, ligase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus : Q9NPD8
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 107240.64

構造登録者

Morreale, F.E.,Testa, A.,Chaugule, V.K.,Bortoluzzi, A.,Ciulli, A.,Walden, H. (登録日: 2017-07-21, 公開日: 2017-10-04, 最終更新日: 2024-01-17)

主引用文献

Morreale, F.E.,Testa, A.,Chaugule, V.K.,Bortoluzzi, A.,Ciulli, A.,Walden, H.
Mind the Metal: A Fragment Library-Derived Zinc Impurity Binds the E2 Ubiquitin-Conjugating Enzyme Ube2T and Induces Structural Rearrangements.
J. Med. Chem., 60:8183-8191, 2017

PubMed Abstract: Efforts to develop inhibitors, activators, and effectors of biological reactions using small molecule libraries are often hampered by interference compounds, artifacts, and false positives that permeate the pool of initial hits. Here, we report the discovery of a promising initial hit compound targeting the Fanconi anemia ubiquitin-conjugating enzyme Ube2T and describe its biophysical and biochemical characterization. Analysis of the co-crystal structure led to the identification of a contaminating zinc ion as solely responsible for the observed effects. Zinc binding to the active site cysteine induces a domain swap in Ube2T that leads to cyclic trimerization organized in an open-ended linear assembly. Our study serves as a cautionary tale for screening small molecule libraries and provides insights into the structural plasticity of ubiquitin-conjugating enzymes.

PubMed: 28933844
DOI: 10.1021/acs.jmedchem.7b01071

実験手法

X-RAY DIFFRACTION (1.85 Å)