5OGK

Crystal structure of a nucleotide sugar transporter with bound nucleotide sugar.

5OGK の概要

• エントリーDOI: 10.2210/pdb5ogk/pdb
• 分子名称: GDP-mannose transporter 1, GUANOSINE-5'-DIPHOSPHATE-ALPHA-D-MANNOSE, (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate (3 entities in total)
• 機能のキーワード: slc35, golgi transporter, nucleotide-sugar, membrane protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 301024.38

構造登録者

Newstead, S.,Parker, J.L. (登録日: 2017-07-13, 公開日: 2017-11-22, 最終更新日: 2024-01-17)

主引用文献

Parker, J.L.,Newstead, S.
Structural basis of nucleotide sugar transport across the Golgi membrane.
Nature, 551:521-524, 2017

PubMed Abstract: Glycosylation is a fundamental cellular process that, in eukaryotes, occurs in the lumen of both the Golgi apparatus and the endoplasmic reticulum. Nucleotide sugar transporters (NSTs) are an essential component of the glycosylation pathway, providing the diverse range of substrates required for the glycosyltransferases. NSTs are linked to several developmental and immune disorders in humans, and in pathogenic microbes they have an important role in virulence. How NSTs recognize and transport activated monosaccharides, however, is currently unclear. Here we present the crystal structure of an NST, the GDP-mannose transporter Vrg4, in both the substrate-free and the bound states. A hitherto unobserved requirement of short-chain lipids in activating the transporter supports a model for regulation within the highly dynamic membranes of the Golgi apparatus. Our results provide a structural basis for understanding nucleotide sugar recognition, and provide insights into the transport and regulatory mechanism of this family of intracellular transporters.

PubMed: 29143814
DOI: 10.1038/nature24464

実験手法

X-RAY DIFFRACTION (3.6 Å)