5OG9

The BM3 mutant WIFI-WC heme domain in complex with testosterone

Summary for 5OG9

• Entry DOI: 10.2210/pdb5og9/pdb
• Descriptor: Bifunctional cytochrome P450/NADPH--P450 reductase, PROTOPORPHYRIN IX CONTAINING FE, ZINC ION, ... (6 entities in total)
• Functional Keywords: cytochrome p450, testosterone, laboratory evolution, hydroxylation, steroids, oxidoreductase
• Biological source: Bacillus megaterium
• Total number of polymer chains: 2
• Total formula weight: 110817.23

Authors

Gamble, C.,Munro, A.W.,Leys, D. (deposition date: 2017-07-12, release date: 2018-08-01, Last modification date: 2024-01-17)

• Primary citation: Acevedo-Rocha, C.G.,Gamble, C.,Lonsdale, R.,Li, A.,Nett, N.,Hoebenreich, S.,Lingnau, J.,Wirtz, C.,Fares, C.,Hinrichs, H.,Deege, A.,Mulholland, A.J.,Nov, Y.,Leys, D.,Munro, A.W.,Reetz, M.T.; Regio- and diastereoselective hydroxylation of steroids using P450-BM3: Readdressing the numbers problem in directed evolution; To Be Published,

Experimental method

X-RAY DIFFRACTION (2.09 Å)