5OEJ

Structure of Tra1 subunit within the chromatin modifying complex SAGA

Summary for 5OEJ

• Entry DOI: 10.2210/pdb5oej/pdb
• EMDB information: 3790 3804
• Descriptor: Tra1 subunit within the chromatin modifying complex SAGA (1 entity in total)
• Functional Keywords: yeast, tra1, saga, activator target, transcription
• Biological source: Komagataella pastoris (Yeast)
• Total number of polymer chains: 1
• Total formula weight: 438055.34

Authors

Sharov, G.,Voltz, K.,Durand, A.,Kolesnikova, O.,Papai, G.,Myasnikov, A.G.,Dejaegere, A.,Ben-Shem, A.,Schultz, P. (deposition date: 2017-07-07, release date: 2017-08-02, Last modification date: 2024-05-15)

Primary citation

Sharov, G.,Voltz, K.,Durand, A.,Kolesnikova, O.,Papai, G.,Myasnikov, A.G.,Dejaegere, A.,Shem, A.B.,Schultz, P.
Structure of the transcription activator target Tra1 within the chromatin modifying complex SAGA.
Nat Commun, 8:1556-1556, 2017

PubMed Abstract: The transcription co-activator complex SAGA is recruited to gene promoters by sequence-specific transcriptional activators and by chromatin modifications to promote pre-initiation complex formation. The yeast Tra1 subunit is the major target of acidic activators such as Gal4, VP16, or Gcn4 but little is known about its structural organization. The 430 kDa Tra1 subunit and its human homolog the transformation/transcription domain-associated protein TRRAP are members of the phosphatidyl 3-kinase-related kinase (PIKK) family. Here, we present the cryo-EM structure of the entire SAGA complex where the major target of activator binding, the 430 kDa Tra1 protein, is resolved with an average resolution of 5.7 Å. The high content of alpha-helices in Tra1 enabled tracing of the majority of its main chain. Our results highlight the integration of Tra1 within the major epigenetic regulator SAGA.

PubMed: 29146944
DOI: 10.1038/s41467-017-01564-7

Experimental method

ELECTRON MICROSCOPY (5.7 Å)