5OEJ

Structure of Tra1 subunit within the chromatin modifying complex SAGA

5OEJ の概要

• エントリーDOI: 10.2210/pdb5oej/pdb
• EMDBエントリー: 3790 3804
• 分子名称: Tra1 subunit within the chromatin modifying complex SAGA (1 entity in total)
• 機能のキーワード: yeast, tra1, saga, activator target, transcription
• 由来する生物種: Komagataella pastoris (Yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 438055.34

構造登録者

Sharov, G.,Voltz, K.,Durand, A.,Kolesnikova, O.,Papai, G.,Myasnikov, A.G.,Dejaegere, A.,Ben-Shem, A.,Schultz, P. (登録日: 2017-07-07, 公開日: 2017-08-02, 最終更新日: 2024-05-15)

主引用文献

Sharov, G.,Voltz, K.,Durand, A.,Kolesnikova, O.,Papai, G.,Myasnikov, A.G.,Dejaegere, A.,Shem, A.B.,Schultz, P.
Structure of the transcription activator target Tra1 within the chromatin modifying complex SAGA.
Nat Commun, 8:1556-1556, 2017

PubMed Abstract: The transcription co-activator complex SAGA is recruited to gene promoters by sequence-specific transcriptional activators and by chromatin modifications to promote pre-initiation complex formation. The yeast Tra1 subunit is the major target of acidic activators such as Gal4, VP16, or Gcn4 but little is known about its structural organization. The 430 kDa Tra1 subunit and its human homolog the transformation/transcription domain-associated protein TRRAP are members of the phosphatidyl 3-kinase-related kinase (PIKK) family. Here, we present the cryo-EM structure of the entire SAGA complex where the major target of activator binding, the 430 kDa Tra1 protein, is resolved with an average resolution of 5.7 Å. The high content of alpha-helices in Tra1 enabled tracing of the majority of its main chain. Our results highlight the integration of Tra1 within the major epigenetic regulator SAGA.

PubMed: 29146944
DOI: 10.1038/s41467-017-01564-7

実験手法

ELECTRON MICROSCOPY (5.7 Å)