5OA1

RNA polymerase I pre-initiation complex

Summary for 5OA1

• Entry DOI: 10.2210/pdb5oa1/pdb
• EMDB information: 3727
• Descriptor: DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (32 entities in total)
• Functional Keywords: rna polymerase i, pre-initiation complex, transcription
• Biological source: Saccharomyces cerevisiae S288C; More
• Total number of polymer chains: 34
• Total formula weight: 944730.95

Authors

Sadian, Y.,Tafur, L.,Kosinski, J.,Jakobi, A.J.,Muller, C.W. (deposition date: 2017-06-20, release date: 2017-07-26, Last modification date: 2025-04-09)

Primary citation

Sadian, Y.,Tafur, L.,Kosinski, J.,Jakobi, A.J.,Wetzel, R.,Buczak, K.,Hagen, W.J.,Beck, M.,Sachse, C.,Muller, C.W.
Structural insights into transcription initiation by yeast RNA polymerase I.
EMBO J., 36:2698-2709, 2017

PubMed Abstract: In eukaryotic cells, RNA polymerase I (Pol I) synthesizes precursor ribosomal RNA (pre-rRNA) that is subsequently processed into mature rRNA. To initiate transcription, Pol I requires the assembly of a multi-subunit pre-initiation complex (PIC) at the ribosomal RNA promoter. In yeast, the minimal PIC includes Pol I, the transcription factor Rrn3, and Core Factor (CF) composed of subunits Rrn6, Rrn7, and Rrn11. Here, we present the cryo-EM structure of the 18-subunit yeast Pol I PIC bound to a transcription scaffold. The cryo-EM map reveals an unexpected arrangement of the DNA and CF subunits relative to Pol I. The upstream DNA is positioned differently than in any previous structures of the Pol II PIC. Furthermore, the TFIIB-related subunit Rrn7 also occupies a different location compared to the Pol II PIC although it uses similar interfaces as TFIIB to contact DNA. Our results show that although general features of eukaryotic transcription initiation are conserved, Pol I and Pol II use them differently in their respective transcription initiation complexes.

PubMed: 28739580
DOI: 10.15252/embj.201796958

Experimental method

ELECTRON MICROSCOPY (4.4 Å)