5O8N

Structure of thermolysin at room temperature via a method of acoustically induced rotation.

Summary for 5O8N

• Entry DOI: 10.2210/pdb5o8n/pdb
• Descriptor: Thermolysin, CALCIUM ION, ZINC ION, ... (6 entities in total)
• Functional Keywords: hydrolase, zinc protease
• Biological source: Bacillus thermoproteolyticus
• Cellular location: Secreted: P00800
• Total number of polymer chains: 1
• Total formula weight: 34807.37

Authors

Axford, D.N.,Burton, C.,Docker, P.,Prince, M.,Topham, P.D. (deposition date: 2017-06-13, release date: 2017-08-09, Last modification date: 2024-01-17)

Primary citation

Burton, C.G.,Axford, D.,Edwards, A.M.J.,Gildea, R.J.,Morris, R.H.,Newton, M.I.,Orville, A.M.,Prince, M.,Topham, P.D.,Docker, P.T.
An acoustic on-chip goniometer for room temperature macromolecular crystallography.
Lab Chip, 17:4225-4230, 2017

PubMed Abstract: This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for gradual in-fluid sample reorientation about varying axes and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facilitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.

PubMed: 29124258
DOI: 10.1039/c7lc00812k

Experimental method

X-RAY DIFFRACTION (1.9 Å)