5O8M

Crystal structure of the MmI1 YTH domain

Summary for 5O8M

• Entry DOI: 10.2210/pdb5o8m/pdb
• Descriptor: YTH domain-containing protein mmi1, GLYCEROL (3 entities in total)
• Functional Keywords: yth domain, meiotic regulator, rna binding protein
• Biological source: Schizosaccharomyces pombe (Fission yeast)
• Cellular location: Nucleus : O74958
• Total number of polymer chains: 4
• Total formula weight: 66503.77

Authors

Brettschneider, J.,Verdel, A.,Kadlec, J. (deposition date: 2017-06-13, release date: 2018-04-25, Last modification date: 2024-05-08)

Primary citation

Touat-Todeschini, L.,Shichino, Y.,Dangin, M.,Thierry-Mieg, N.,Gilquin, B.,Hiriart, E.,Sachidanandam, R.,Lambert, E.,Brettschneider, J.,Reuter, M.,Kadlec, J.,Pillai, R.,Yamashita, A.,Yamamoto, M.,Verdel, A.
Selective termination of lncRNA transcription promotes heterochromatin silencing and cell differentiation.
EMBO J., 36:2626-2641, 2017

PubMed Abstract: Long non-coding RNAs (lncRNAs) regulating gene expression at the chromatin level are widespread among eukaryotes. However, their functions and the mechanisms by which they act are not fully understood. Here, we identify new fission yeast regulatory lncRNAs that are targeted, at their site of transcription, by the YTH domain of the RNA-binding protein Mmi1 and degraded by the nuclear exosome. We uncover that one of them, , regulates entry into sexual differentiation. Importantly, we demonstrate that Mmi1 binding to this lncRNA not only triggers its degradation but also mediates its transcription termination, thus preventing lncRNA transcription from invading and repressing the downstream gene encoding a mitogen-activated protein kinase kinase kinase (MAPKKK) essential to sexual differentiation. In addition, we show that Mmi1-mediated termination of lncRNA transcription also takes place at pericentromeric regions where it contributes to heterochromatin gene silencing together with RNA interference (RNAi). These findings reveal an important role for selective termination of lncRNA transcription in both euchromatic and heterochromatic lncRNA-based gene silencing processes.

PubMed: 28765164
DOI: 10.15252/embj.201796571

Experimental method

X-RAY DIFFRACTION (1.45 Å)