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5O87

Crystal structure of wild type Aplysia californica AChBP in complex with nicotine

Summary for 5O87
Entry DOI10.2210/pdb5o87/pdb
DescriptorSoluble acetylcholine receptor, (S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
Functional Keywordsreceptor, signaling protein
Biological sourceAplysia californica (California sea hare)
Total number of polymer chains10
Total formula weight292209.13
Authors
Dawson, A.,Hunter, W.N.,de Souza, J.O.,Trumper, P. (deposition date: 2017-06-12, release date: 2018-06-27, Last modification date: 2024-10-23)
Primary citationDawson, A.,Trumper, P.,de Souza, J.O.,Parker, H.,Jones, M.J.,Hales, T.G.,Hunter, W.N.
Engineering a surrogate human heteromeric alpha / beta glycine receptor orthosteric site exploiting the structural homology and stability of acetylcholine-binding protein.
Iucrj, 6:1014-1023, 2019
Cited by
PubMed Abstract: Protein-engineering methods have been exploited to produce a surrogate system for the extracellular neurotransmitter-binding site of a heteromeric human ligand-gated ion channel, the glycine receptor. This approach circumvents two major issues: the inherent experimental difficulties in working with a membrane-bound ion channel and the complication that a heteromeric assembly is necessary to create a key, physiologically relevant binding site. Residues that form the orthosteric site in a highly stable ortholog, acetylcholine-binding protein, were selected for substitution. Recombinant proteins were prepared and characterized in stepwise fashion exploiting a range of biophysical techniques, including X-ray crystallography, married to the use of selected chemical probes. The decision making and development of the surrogate, which is termed a glycine-binding protein, are described, and comparisons are provided with wild-type and homomeric systems that establish features of molecular recognition in the binding site and the confidence that the system is suited for use in early-stage drug discovery targeting a heteromeric α/β glycine receptor.
PubMed: 31709057
DOI: 10.1107/S205225251901114X
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.2 Å)
Structure validation

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