5O7T

Crystal structure of KlenTaq mutant M747K in a closed ternary complex with a dG:dCTP base pair

5O7T の概要

• エントリーDOI: 10.2210/pdb5o7t/pdb
• 分子名称: DNA polymerase I, thermostable, DNA primer, DNA template, ... (7 entities in total)
• 機能のキーワード: dna polymerase, transferase
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 70148.70

構造登録者

Betz, K.,Diederichs, K.,Marx, A. (登録日: 2017-06-09, 公開日: 2017-11-29, 最終更新日: 2024-01-17)

主引用文献

Betz, K.,Nilforoushan, A.,Wyss, L.A.,Diederichs, K.,Sturla, S.J.,Marx, A.
Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase.
Chem. Commun. (Camb.), 53:12704-12707, 2017

PubMed Abstract: The possibility to sequence cytotoxic O-alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing.

PubMed: 29136072
DOI: 10.1039/c7cc07173f

実験手法

X-RAY DIFFRACTION (1.8 Å)